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If creating v2 sample sheets for devices aside from those detailed, ensure the final sample sheet has the i5 index in the proper orientation as described earlier mentioned.

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If working in handbook manner and working with bcl2fastq or BCL Change, or uploading the sample sheet to BaseSpace as being a handbook manner run and attaching a sample sheet, enter the i5 sequence in the ahead route.

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Established C Index Adapters Confer with Index two (i5) Orientation For more info on how to click here enter i5 bases within the sample sheet in forward or reverse enhance orientation.

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When working with BaseSpace Sequence Hub PrepTab, i5 sequences has to be entered into these software while in the ahead orientation in all conditions and for all instrument types.

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